Laboratory of molecular genetic research

Focus area of the laboratory

— development and further use in the breeding of systems of molecular markers for selecting sunflower genotypes with complex resistance to oil crops pathogens, which will increase the efficiency of breeding in developing lines and hybrids resistant to a complex of pathogens;

— development and further use in the breeding of DNA-marker systems to identify the genes that control the important breeding traits of oil crops, which will increase the efficiency of breeding in developing new genotypes with a given set of economically important traits;

— development of new and optimization of existing systems of DNA markers for molecular-genetic identification and certification of breeding samples, lines, hybrids, and varieties of sunflower, soybean, flax, and rapeseed from the collection of V. S. Pustovoit All-Russian Research Institute of Oil Crops, which will allow studying the molecular genetic diversity of these crops, to estimate the genetic distance between genotypes for selection of parent pairs in hybridization, to certify modern varieties for their further identification, determination of typicality and copyright protection;

— development of molecular genetic passports for new lines and hybrids of sunflower, soybean, flax, and rapeseed varieties of the collection of V. S. Pustovoit All-Russian Research Institute of Oil Crops based on molecular genetic markers.

Laboratory results for 2019-2022

— dominant and codominant molecular genetic markers of mutation of high oleic content were identified and validated for lines of V. S. Pustovoit All-Russian Research Institute of Oil Crops with different content of oleic acid in sunflower seed oil;

— promising DNA markers were found to identify Pl6, Pl8, and Plarg genes controlling the resistance of sunflower to downy mildew pathogen in the collection of lines of V. S. Pustovoit All-Russian Research Institute of Oil Crops;

— we excluded the possibility to locate the gene Or7 controlling resistance of sunflower to race G of broomrape in close proximity to SSR loci ORS 683, ORS 1040, and ORS 1112 on chromosome 3. The region of a further search for the location of the Or7 gene on the linkage map was determined;

— the system of microsatellite markers for genotyping oil flax varieties of breeding of V. S. Pustovoit All-Russian Research Institute of Oil Crops was developed;

— the genetic structure of 4 promising oil flax varieties has been determined using 12 polymorphic microsatellite loci;

— the systems of microsatellite markers for genotyping sunflower and soybean lines and hybrids are optimized, including the use of fragment analysis by capillary electrophoresis;

— about 150 lines, hybrids, and varieties of sunflower, soybean, and oil flax were genotyped by microsatellite markers and the degree of their genetic diversity was determined;

— the work on the development of a microsatellite loci system for genotyping of rapeseed of breeding of V. S. Pustovoit All-Russian Research Institute of Oil Crops has been started. Optimal amplification regimes were determined for the genotyping of rapeseed lines and hybrids using 7 primers for 20 rapeseed lines.

Publications

1. S. A. Ramazanova, T. S. Antonova. To a question about marking of Pl loci controlling sunflower resistance to downy mildew pathogen // Oil crops. — 2019. — № 1(177). — P. 17-23.

2. T. A. Chelyustnikova, S. Z. Guchetl, T. S. Antonova. Microsatellite loci for identification of oil flax varieties of the breeding of V. S. Pustovoit All-Russian Research Institute of Oil Crops: selection of informative primers and optimal conditions for DNA PCR // Oil crops. — 2019. — № 2(178). — P. 41-46.

3. S. A. Ramazanova, T. S. Antonova, E. V. Badyanov, S. V. Syksin, P. Y. Merkulov. Usage of SNP-markers for identification of loci Pl5 and PL8 controlling sunflower resistance to Plasmopara halstedii (Farl.) Berl. et de Toni // Oil crops. — 2019. — № 3(179). — P. 21-26.

4. S. Z. Guchetl, T. A. Chelyustnikova. Development of methods for identifying oil flax varieties of the breeding of V. S. Pustovoit All-Russian Research Institute of Oil Crops using DNA-markers // «Innovative technologies of domestic breeding and seed production: collection of abstracts on materials of the III scientific-practical conference of young scientists of All-Russian forum on breeding and seed production conference «Russian Field», October 16-17, 2019 — Krasnodar: KubSAU, 2019. — P. 93-95.

5. E. V. Badyanov, S. A. Ramazanova. Marking of Pl5 and Pl8 genes controlling sunflower resistance to Plasmopara halstedii (Farl.) Berl. Et de Toni // «Innovative technologies of domestic breeding and seed production: collection of abstracts on materials of the III scientific-practical conference of young scientists of All-Russian forum on breeding and seed production conference «Russian Field», October 16-17, 2019 — Krasnodar: KubSAU, 2019. — P. 96-98.

6. E. V. Badyanov, S. A. Ramazanova. Identification of Pl5, Pl6 and Pl8 DNA loci controlling resistance to Plasmopara halstedii in sunflower lines // Current issues of biology, breeding, cultivation and processing technology of oil and other technical crops. Proceedings of the 10th All-Russian conference with international participation of young scientists and specialists. — 2019. — P. 12-16.

7. S. Z. Guchetl, T. S. Antonova, N. M. Araslanova, T. A. Chelyustnikova. Analysis of the identity of resistance genes to race G of broomrape in some lines of sunflower in F1 // Agrarian Science. — 2019. — № 9. — P. 43-48.

8. S. Z. Guchetl, T. S. Antonova, N. M. Araslanova, T. A. Chelyustnikova, Yu. V. Pitinova. Genetic analysis of resistance to race G of Orobanche cumana Wallr. in F2 and BC1 of sunflower lines RGP1, RGP2, RGВ, RGL1, RGL2 // Oil crops. — 2019. — № 4(180). — P. 23-28.

9. Guchetl S., Antonova T., Araslanova N., Tchelyustnikova T. Sunflower resistance to race G of broomrape (Orobanche cumana Wallr.) in the Russian Federation: the development of the lines and the study of inheritance // Helia. — Т. 42. — No 71. — P. 161–171.

10. S. V. Zelentsov, E. V. Moshnenko, G. M. Saenko, M. V. Trunova, A. A. Tkachyova, E. N. Budnikov, L. A. Bubnova, S. A. Ramazanova. A middle-early maturing soybean cultivar Bars // Oil crops. — 2019. — № 1(177). — P. 138-142.

11. S. V. Zelentsov, E. V. Moshnenko, L. A. Bubnova, E. N. Budnikov, M. V. Trunova, A. A. Tkachyova, G. M. Saenko, S. A. Ramazanova. A middle-early maturing cold-resistant soybean cultivar Iney // Oil crops. — 2019. — № 3(179). — P. 160-164.

12. S. A. Ramaanova, A. S. Kolomytseva. Optimization of soybean genotyping process using analysis of a polymorphism of SSR-loci in DNA // Oil crops. — 2020. — № 3(181). — P. 42-48.

13. S. Z. Guchetl. Dominant molecular markers of mutation of high oleic oil in sunflower seeds // Oil crops. — 2020. — № 2(182). — P. 24-32.

14. V. A. Mnatsakanova, A. V. Golovatskaya, D. L. Savichenko, S. Z. Guchetl, N. A. Pikalova. Polymorphism of microsatellite loci of sunflower resistant and susceptible to race E of broomrape // Proceedings of the XXVII International scientific conference of students, postgraduates and young scientists «Lomonosov-2020», April 22-24, 2020, Sevastopol / Edited by O. A. Shpyrko. — 2020. — P. 25.

15. V. A. Mnatsakanova, D. L. Savichenko, S. Z. Guchetl, N. A. Pikalova, A. Kh. Guchetl. Linkage analysis of the resistance gene Or5 of sunflower broomrape with SSR DNA locus // Lomonosov-2020: Proceedings of the XXVII International scientific conference of students, postgraduates and young scientists «Lomonosov-2020», April 22-24, 2020, Sevastopol / Edited by O. A. Shpyrko. — 2020. — P. 24.

16. D. L. Savichenko, S. Z. Guchetl, A. V. Golovatskaya. Search and analysis of nuclear sequences in sunflower genome associated with genes of resistance to broomrape (Orobanche cumana Wallr.) races // Oil crops. — 2020. — № 3(183). — P. 11-19.

17. S. A. Ramazanova, E. V. Badyanov, S. Z. Guchetl. Molecular markers of genes Pl6, Pl13 and Plarg for sunflower breeding on resistance to downy mildew // Oil crops. — 2020. — № 3(183). — P. 20-26. 18. S. Z. Guchetl, T. A. Chelyustnikova. Genotyping of oil flax varieties using the system of microsatellite DNA markers // Agrarian Science of the Euro-North-East. — 2020. — V. 21. — № 5. — P. 531-539.

19. N. M. Araslanova, S. Z. Guchetl, T. A. Chelyustnikova, T. S. Antonova, Yu. V. Pitinova. Description of sunflower lines resistant to race G of broomrape // Oil crops. — 2020. — № 1(181). — P. 38-41.

20. S. Z. Guchetl, T. S. Antonova, N. M. Araslanova, T. A. Chelyustnikova, Yu. V. Pitinova. Inheritance of resistance of sunflower RGM line to race G of broomrape in F1 // Proceedings of Kuban State Agrarian University. — 2020. — № 82. — P. 63-67.

21. A. V. Golovatskaya, S. Z. Guchetl. Certification of sunflower lines from the collection of DES of V. S. Pustovoit All-Russian Research Institute of Oil Crops by DNA markers // Current issues of biology, breeding, cultivation and processing technology of agricultural crops: Proceedings of the 11th All-Russian conference of young scientists and specialists. — Krasnodar, 2021. — P. 39-43.

22. D. L. Savichenko, S. Z. Guchetl. Problem of detection of heterozygous genotypes by mutation of FAD 2-1 gene of sunflower (Helianthus annuus L.) using DNA-markers // Current issues of biology, breeding, cultivation and processing technology of agricultural crops: Proceedings of the 11th All-Russian conference of young scientists and specialists. — Krasnodar, 2021. — P. 102-106.

23. A. A. Averina, T. A. Chelyustnikova, S. Z. Guchetl. Search and selection of promising microsatellite loci for molecular-genetic certification of oil flax collection of V. S. Pustovoit All-Russian Research Institute of Oil Crops // Current issues of biology, breeding, cultivation and processing technology of agricultural crops: Proceedings of the 11th All-Russian conference of young scientists and specialists. — Krasnodar, 2021. — P. 7-11.

24. E. V. Badyanov, S. A. Ramazanova. Testing SSR-markers of sunflower genes controlling resistance to P. halstedii and selection of optimal conditions for PCR // Current issues of biology, breeding, cultivation and processing technology of agricultural crops: Proceedings of the 11th All-Russian conference of young scientists and specialists. — Krasnodar, 2021. — P. 19-24.

25. V. G. Savichenko, S. A. Ramazanova. Identification of soybean varieties of the breeding of V. S. Pustovoit All-Russian Research Institute of Oil Crops by microsatellite analysis // Current issues of biology, breeding, cultivation and processing technology of agricultural crops: Proceedings of the 11th All-Russian conference of young scientists and specialists. — Krasnodar, 2021. — P. 97-101.

26. Ramazanova S. A., Savichenko V. G., Guchetl S. Z. Genetic analysis of the homogeneity of soybean varieties by microsatellite loci // In: IOP Conference series: earth and environmental science. International Conference on Production and Processing of Agricultural Raw Materials. — 2021. — Art. 012007.

27. Guchetl S. Z., Savichenko D. L., Ramazanova S. A. The search for the location of the sunflower resistance gene to race G of broomrape on the linkage map in the lines of breeding of VNIIMK // In: IOP Conference series: earth and environmental science. International Conference on Production and Processing of Agricultural Raw Materials. — 2021. — Art. 012008.

28. S. Z. Guchetl, M. L. Zolotavina, A. A. Grigoryan, A. V. Golovatskaya. Quality of DNA for PCR extracted from sunflower with different methods // Oil crops. — 2021. — № 1(185). — P. 32-42.

29. S. Z. Guchetl, D. L. Savichenko. A linkage of gene of resistance to a broomrape race G with microsatellite loci of a sunflower line donor RGP1 of VNIIMK’s breeding // Oil crops. — 2021. — № 2(186). — P. 3-9.

30. S. V. Zelentsov, E. V. Moshnenko, M. V. Trunova, L. A. Bubnova, E. N. Budnikov, G. M. Saenko, S. A. Ramazanova. An early cold- and drought-resistant soybean variety Triada // Oil crops. — 2021. — № 2(186). — P. 92-97.

31. S. V. Zelentsov, E. V. Moshnenko, M. V. Trunova, L. A. Bubnova, E. N. Budnikov, A. V. Lukomets, V. G. Savichenko. A cold-resistant soybean cultivar of the northern ecotype Sayana // Oil crops. — 2021. — № 1(185). — P. 95-102.

32. A. V. Golovatskaya, S. Z. Guchetl. Certification and identification of sunflower hybrids from the collection of DES of V. S. Pustovoit All-Russian Research Institute of Oil Crops using SSR-markers // Proceedings of the International scientific and practical conference «Scientific support for sustainable development of agroindustrial complex under aridizing climate», dedicated to the 35th anniversary of FGBNU RosNIISC «Rossorgo», 25-26 March 2021. — P. 91-96.

33. V. G. Savichenko, S. A. Ramazanova. Determination of intravarietal polymorphism of soybean varieties by microsatellite (SSR) loci // Proceedings of the International scientific and practical conference «Scientific support for sustainable development of agroindustrial complex under aridizing climate», dedicated to the 35th anniversary of FGBNU RosNIISC «Rossorgo», 25-26 March 2021. — P. 232-238.

34. S. A. Ramazanova, V. G. Savichenko, E. G. Ustarkhanova, E. D. Loginova, R. N. Ramazanov, A. Kh. Guchetl. Search of the new SSR-loci of DNA for development of effective technology for soybean genotyping // Oil crops. — 2021. — № 4(188). — P. 18-24.

35. N. M. Araslanova, T. S. Antonova, S. Z. Guchetl, T. A. Chelyustnikova, Yu. V. Pitinova. Morphological traits of new sunflower lines resistant to broomrape race G // Oil crops. — 2021. — № 2(186). — P. 18-23.

36. S. Z. Guchetl, T. S. Antonova, N. M. Araslanova, T. A. Chelyustnikova, Yu. V. Pitinova. Identity of genes of resistance to broomrape race G in sunflower lines of different origin // Oil crops. — 2021. — № 3(187). — P. 3-9.

37. S. Z. Guchetl, T. S. Antonova, N. M. Araslanova, T. A. Chelyustnikova, Yu. V. Pitinova. Molecular-genetic diversity of the collection of 14 sunflower lines — donors of resistance to broomrape race G based on isoenzymes and SSR-loci // Oil crops. — 2022. — № 1(189). — P. 3-10.

38. Ramazanova S. A., Badyanov E. V., Guchetl S. Z. Validation of microsatellite markers to identify Pl6, Pl8 and Plarg genes that control resistance to Plasmopara halstedii in sunflower // Caspian J. Environ. Sci. — 2021. — V. 19. — I. 5. — P. 915–220.

39. S. Z. Guchetl, T. A. Chelyustnikova, A. A. Averina. Analysis of oil flax varieties’ structure based on polymorphic microsatellite loci // Agrarian Science of the Euro-North-East. — 2022. — 23 (2). — P. 184-193.

40. T. A. Chelyustnikova, A. A. Averina, S. Z. Guchetl, M. L. Zolotavina, S. A. Ramazanova, A. A. Voloshko, E. D. Loginova. Optimization of marker system for genotyping oil flax varieties from the collection of V. S. Pustovoit All-Russian Research Institute of Oil Crops // Agrarian Science. — 2022. — № 4. — P. 57-61.

41. S. A. Ramazanova, E. V. Badyanov, V. G. Savichenko, S. V. Ivanov, A. A. Averina. Microsatellite markers for identification of gene Plarg controlling resistance to downy mildew in sunflower // Proceedings of International scientific and practical conference. «Ecological and genetic basis of breeding and cultivation of agricultural crops», Krasnodar, May 24-27, 2022.

Information about the received patents

T. S. Antonova, N. M. Araslanova, S. Z. Guchetl, T. A. Chelyustnikova. Antonova T. S., Araslanova N. M., Guchetl S. Z., Chelyustnikova T. A. Method of accelerating the production of a donor line of sunflower resistance to broomrape race G (Orobanche cumana Wallr.). Patent for the invention 2720915 C1, 14.05.2020. Application no. 2019124249, 26.07.2019.

Grants

1. Grant of Kuban Scientific Foundation, the project Nastavnik-21. 1: «Molecular labeling of Plarg gene controlling resistance to downy mildew Plasmopara halstedii (Farl.) Berl. et de Toni. in sunflower». Supervisor Ramazanova S. A.

2. Participation together with the Laboratory of immunity in the scientific project 19-44-230025: «Genetic control of resistance to broomrape race G in sunflower lines of different origin» supported by the grant of the Russian Foundation for Basic Research and the Administration of the Krasnodar region. Participants: Guchetl S. Z., Chelyustnikova T. A.

3. Participation in the scientific project 19-416-235001 r_Nastavnik: «Genetic characteristics of the trait of medium oleic content of oil in sunflower seeds» supported by the grant of the Russian Foundation for Basic Research and the Administration of the Krasnodar region. Participant: Savichenko D. L.