The laboratory of biochemistry

Efimenko Sergey Grigorievich

Efimenko Sergey Grigorievich

Head of the laboratory of biochemistry
PhD in biology

Tel.: (861) 274-53-94
Fax: (861) 254-27-80
E-mail: efimenko-km@yandex.ru

The laboratory of biochemistry, reorganized in the 30s into the department, was established in 1922-1923 by the famous biochemist Sergey V. Rushkovsky (1886-1960).

S.V. Rushkovsky was in charge of the biochemical department for 25 years. He developed highly efficient method of quantitative estimation of oil in the sunflower seeds, which provided great success in the breeding of this crop for high oil content of seeds.

In 1947-1969 the department was headed by N.F. Dublyanskaya, in 1970-1986 - by A.A. Borodulina, in 1986-1990 - by P.S. Popov, and in 1990-1991 - by Ya.N. Demurin.

In 1992, the biochemistry department had merged with the central analytical research laboratory (CARL) with simultaneous formation of the laboratory of biochemical genetics. In 1992-2004 the department of biochemistry was headed by I.V. Shvedov.

Highly qualified scientific personnel have been working in the department since its foundation. They defended 16 PhD and two doctoral theses, published a few hundreds of scientific articles, received 29 certificates and patents for inventions, wrote a lot of guidance papers on the invention level.

The laboratory's employees have mastered methods of determination of fatty acid compositions using the gas-liquid chromatograph Kristall 2000M with a capillary column (more than 30 fatty acids were identified), tocopherols and phospholipids composition by using method of thin-layer chromatography with an automatic applicator, total content of tocopherols (vitamin E), carotenoids (vitamin A). The technique of determining the oxidative stability of oil by using the device RANCIMAT 743 under the temperature of 80-220 0C was also mastered.

Each year, the laboratory carries out analyses on the content of fatty acids (4.5 thousands), on the content of glucosinolates and essential oil (3.1 thousands), complex research of 820 samples of soybean seeds on the IR-analyzer NIR System 4500 (protein, oil content, trypsin-inhibiting activity (TIA) , and 150 analyses of acid number in the seed oil.